The ship is carrying 629 rescued migrants, who joined together in early morning prayers on board the Aquarius as Malta and Italy stood their ground.
The European Union and the United Nations refugee agency called for a swift end to a political standoff that has left 629 migrants on a rescue ship drifting at sea after Italy refused to let it dock.
Some 629 people, including pregnant women and scores of children, were saved by SOS Mediterranean on Saturday and are stuck aboard the French NGO's ship Aquarius, which is now between Malta and Sicily waiting for a secure port.
Maltese Prime Minister Joseph Muscat said Italy's refusal to allow the Aquarius ship to dock at an Italian port risked "creating a risky situation for all those involved".
Matteo Salvini, the head of the far-right League party who became interior minister this month vowing to crack down on the influx of migrants from Africa, blocked the ship, operated by SOS Mediterranee and Doctors without Borders, over the weekend.
Tweeting on Sunday, Salvini said: "Starting today, Italy, too, begins to say NO to the trafficking of human beings, NO to the business of clandestine immigration".
Refugees and migrants are transferred to the SOS Mediterranee Aquarius rescue ship a day after being rescued by members of the Spanish NGO Proactiva Open Arms on Monday, May 7, 2018. Salvini had earlier issued a joint statement with Italian Transportation Minister Danilo Toninelli of the 5-Star Movement, saying Malta "cannot continue to look the other way when it comes to respecting precise global conventions on the protection of human life".
Meanwhile a spokesman for the Maltese government said that "Malta was neither the coordinating nor the competent authority" in the rescue.
Italian Premier Giuseppe Conte said on Facebook that he had called on his Maltese counterpart, Joseph Muscat, to "explicitly at least take on the human assistance of persons in difficulty aboard the Aquarius".
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The League promisied voters during the Italian general election that it would take a tough stance on immigration.
Mr Salvini says he wants to "stop the death boats".
"Our sole objective is to bring the people we've rescued, in hard conditions yesterday, to a port of safety", the group said in a statement.
More than 550,000 migrants have arrived in Italy between June 2014 and June 2017.
The mayors of Palermo, Taranto, Messina and Reggio Calabria, have also said they would be ready to welcome the vessel.
SOS Mediterranee said the crew of the Aquarius were still at sea and awaiting instructions.
The closest, safest port, excluding Libya, was Lampedusa, however, Italian Home Affairs Minister Matteo Salvini ordered all of his country's ports closed to the ship.
It also said it requested help from the coastguard in Malta to send boats to aid the rescue mission but Malta refused.
The kids also complained they were hot during the drive, while being kept in a confined space in the trunk, according to WREG . The video went viral, and eventually led to the arrest of 62-year-old Leimome Cheeks , reported the Commercial Appeal .
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During his preparation period, Trump continued on Monday to publicly brawl on Twitter with USA allies over trade. However, he took a U-turn soon and said the White House was proceeding with the preparations for the summit.
Canadian Prime Minister Justin Trudeau has vowed retaliatory action next month over United States tariffs on steel and aluminium. The Canadian leader pulled a "sophomoric political stunt for domestic consumption", Kudlow said, that amounted to "a betrayal".
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